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Ashwitha, K.
- Characterization and Evaluation of Two Indigenous Bacillus thuringiensis Isolates against Helicoverpa armigera Hubner
Abstract Views :267 |
PDF Views:194
Authors
R. Rangeshwaran
1,
K. Veenakumari
1,
Pritam Karmakar
1,
K. Ashwitha
1,
G. Sivakumar
1,
Satendar Kumar
1
Affiliations
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 4 (2011), Pagination: 286-293Abstract
Two isolates of Bacillus thuringiensis isolated from dead lepidopteran larvae from a tea garden in Jorhat, Assam and one isolated from soil sample from Rajasthan, obtained in a nationwide screening program showed bipyramidal crystal morphology. These two isolates named as NBAII-BTAS and NBAII-BTG4 were characterized by their high level of toxicity against diamond back moth (Plutella xylostella). The PCR amplification of these two isolates revealed the expected size of the PCR product for cry1Aa, cry1Ab, cry1Ac, cry1E, cry1G, cry1I, and cry2 of 390 bps, 1111bps, 238 bps, 540 bps, 300 bps, 468 bps, and 1170 bps respectively. Purified cry proteins from each of these two cultures were subjected to SDS-PAGE analysis, where, two distinct bands of 130–140 Kda and 65 Kda corresponding to cry1 and cry2 proteins were observed. Toxicity studies was carried out using trypsin activated purified proteins against Helicoverpa armigera, where NBAII-BTG4 derived crystal proteins displayed more toxicity (0.93µg\ml) than NBAII-BTAS.Keywords
Cry Genes, PCR, Specific Cry Primers, Bipyramidal, Toxicity.Full Text
References
- Aly, N. A. H. 2007. PCR detection of cry genes in local Bacillus thuringiensis Isolates, Australian Journal of Basic and Applied Sciences, 1: 461–466.
- Anitha, D., Senthil Kumar, N., Vijayan, D., Kunhikrishnan., Ajithkumar and Gurusubramanian, G. 2011. Characterization of Bacillus thuringiensis isolates and their differential toxicity against Helicoverpa armigera populations. Journal of Basic Microbiology, 51: 107–114.
- Avilla, C., Vargas-Osuna, E., González-Cabrera, J., Ferré, J. and González-Zamora, J. E. 2005. Toxicity of several ä-endotoxins of Bacillus thuringiensis against Helicoverpa armigera (Lepidoptera: Noctuidae) from Spain. Journal of Invertebrate Pathology, 90: 51–54.
- Avilla. C., Vargas-Osuna, E., González-Cabrera, J., Ferré, J. and González-Zamora, J. E. 2005. Toxicity of several endotoxins of Bacillus thuringiensis against Helicoverpa armigera (Lepidoptera: Noctuidae) from Spain. Journal of Invertebrate Pathology, 90: 51–54.
- Ben-Dov, E., Wang, Q., Zaritsky, A., Manasherob, R., Barak, Z., Schneider, B., Khamraev, A., Baizhanov, M., Glupov, V. and Margalith, Y. 1999. Multiplex PCR screening to detect cry 9 genes in Bacillus thuringiensis strain. Applied and Environmental Microbiology, 65: 3714–3716.
- Beron, C. M., Curatti, L. and Salerno, G. L. 2005. New strategy for identification of novel cry type genes from Bacillus thuringiensis strains. Applied and Environmental Microbiology, 71: 761–765.
- Beron, C. M. and Salerno, G. L. 2006. Characterization of Bacillus thuringiensis isolates from Argentina that are potentially useful in insect pest control. Biological Control, 51: 779–794.
- Bravo A., Sarabia, S., Lopez, L., Ontiveros, H., Abarca, C., Ortiz, A., Ortiz, M., Lina, L., Villalobos, F., Peña, G., Nuñez-Valdez, M. E., Soberón, M and Quintero, R. 1998. Characterization of cry genes in a mexican Bacillus thuringiensis strain collection. Applied and Environmental Microbiology, 64: 4965–4972.
- Carozzi, N. B., Kramer, V. C., Warren, G. W., Evola, S. and Koziel, M. G. 1991. Prediction of insecticidal activity of Bacillus thuringiensis strains by polymerase chain reaction product profiles. Applied and Environmental Microbiology, 57: 3057–3061.
- Cerón, J. L., Covarrubias, R., Quintero, A., Ortiz, M., Ortiz, E. Aranda and Bravo, A. 1994. PCR analysis of the cry I insecticidal crystal family genes from Bacillus thuringiensis. Applied and Environmental Microbiology, 60: 353–356.
- Cerón, J. A., Ortiz, R., Quintero, L. G. and Bravo, A. 1995. Specific PCR primers directed to identify cryI and cry III genes within a Bacillus thuringiensis stray collection. Applied and Environment Microbiology, 61: 3826–3831.
- Chilcott, C. N. and Wigley, P. J. 1994. Isolation and toxicity of Bacillus thuringiensis from soil and insect habitats in New Zealand. Journal of Invertebrate Pathology, 61: 244–247.
- Gill, S. S., Cowles, E. A. and Pietrantinio, P. V. 1992. The mode of action of Bacillus thuringiensis endotoxins. Annual Review of Entomology, 37: 615–636.
- Höfte H. and Whiteley H. R., 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiological Reviews, 53: 242–255.
- Hofte, H., Van Rie, J., Jansens, S., Van Houtven, A., Vanderbruggen, H. and Vaeck, M. 1988. Monoclonal antibody analysis and insecticidal spectrum of three types of lepidopteran specific insecticidal crystal proteins of Bacillus thuringiensis. Applied and Environmental Microbiology, 54: 2010–2017.
- Konecka, E., Kaznowski, A., Zeimnicka, J., Zeminicki, K. and Paetz, H. 2007. Analysis of cry gene profiles in Bacillus thuringiensis strains isolated during epizootics in Cydia pomonella L., Current Microbiology, 55: 217–222.
- Kronstad, J. W. and Whiteley, H. R. 1986. Three classes of homologous Bacillus thuringiensis crystal protein genes. Gene, 43: 29–40.
- Laemmli, U. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, 227: 680–685.
- Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with the folin phenol reagent. Journal of Biology and Chemistry, 193: 26.
- Mahadi, N. M., Hastowo, S., Lay, B. and Dean, D. H. 1998. Application of Multiplex P.C.R. for rapid determination of cryI gene profiles of new Bacillus thyringiensis isolates. Journal of Microbiology and Biotechnology, 8: 517–522.
- Martin, P. A. W., Travers, R. S. 1989. Worldwide abundance and distrbution of Bacillus thuringiensis isolates. Applied Environmental Microbiology, 55: 2437–2442.
- Morris, O. N., Converse, V., Kanagaratnam, P. and Cote, J. C. 1998. Isolation, characterization and culture of Bacillus thuringiensis from soil and dust from grain storage bins and their toxicity for Mamestra configurata (Lepidoptera: Noctuidae). Canadian Entomologist, 130: 515–537.
- Obeidat, M., Hassawi, D. and Ghabeish I. 2004. Characterization of Bacillus thuringiensis strains from Jordan and their toxicity to the Lepidoptera Ephestia kuehniella Zeller. African Journal of Biotechnology, 3: 622–626.
- Ohba, M. and Aizawa, K. 1986. Distribution of Bacillus thuringiensis in soils of Japan. Journal of Invertebrate Pathology, 47: 277–282.
- Porcar, M. and Jua.rez/Perez, V. 2003. PCR-based identification of Bacillus thuringiensis pesticidal crystal geners. FEMS Microbiology Reviews, 26: 419–432.
- Rosas-Garcia, N. M., Mireles-Martinez, M., Hernandez-Mendoza, J. L. and Ibarra, J. E. 2007. Screening of cry gene contents of Bacillus thuringiensis strains isolated from avocado orchards in Mexico, and their insecticidal activity towards Argyrotaenia sp. (Lepidoptera:Tortricidae) larvae. Journal of Applied Microbiology, 104: 224–230.
- Sambrook, J., Fritsch, E. F. and Mariatis, T. 1989. Molecular cloning: A Laboratory Manual, 2nd Ed., N. Y., Cold Spring Harbor Laboratory Press, 659p.
- Smith, R. A. and Couche, G. A. 1991. The phylloplane as a source of Bacillus thuringiensis. Applied and Environmental Microbiology, 57: 311–315.
- Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Fietelson, J., Ziegler, D. R. and Dean, D. H. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiology and Molecular Biology Reviews. 62: 775–806.
- Shang Kuo, W., Lin, J. H., Tzeng, C. C., Kao, S. S. and Chak, K. F. 2000. Cloning of two new cry Genes from Bacillus thuringiensis subsp. wuhanensis strain. Current Microbiology, 40: 227–232.
- Thammasittirong, A. and Attathom, T. 2008. PCR-based method for the detection of cry genes inlocal isolates of Bacillus thuringiensis from Thailand. Journal Invertebrate Pathology, 98: 121–126.
- Travers, R. S., Martin, P. A. and Reichelderfer, C. F. 1987. Selective process for efficient isolation of soil Bacillus spp. Applied and Environmental Microbiology, 53: 1263- 1266
- Characterization of Abiotic Stress Tolerant Pseudomonas Spp. Occurring in Indian Soils
Abstract Views :375 |
PDF Views:128
Authors
K. Ashwitha
1,
R. Rangeshwaran
1,
N. V. Vajid
2,
G. Sivakumar
1,
S. K. Jalali
1,
K. Rajalaksmi
3,
H. Manjunath
4
Affiliations
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
2 Department of Biology, University of Hail, Jeddah, SA
3 Department of Plant Pathology, University of Agricultural Sciences, Dharwad 580 005, Karnataka, IN
4 Department of Plant Pathology, GKVK, UAS Bangalore 560 065, IN
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
2 Department of Biology, University of Hail, Jeddah, SA
3 Department of Plant Pathology, University of Agricultural Sciences, Dharwad 580 005, Karnataka, IN
4 Department of Plant Pathology, GKVK, UAS Bangalore 560 065, IN
Source
Journal of Biological Control, Vol 27, No 4 (2013), Pagination: 319–328Abstract
Abiotic stress tolerance of 230 Pseudomonas spp. Occurring in Indian soils was evaluated for tolerance to temperature, salinity and moisture stresses. Forty seven Pseudomonas spp. Were characterized as abiotic stress tolerant and were identified as P. aeruginosa (24), P. putida (14), P. plecoglossicida (4), P. mosselli (1), Pseudomonas sp. (1) and P. fluorescens (3). The temperature and salinity tolerance of these bacteria was 45°C and 1 M NaCl respectively. Most isolates (44 out of 47) produced indole acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity and 37 of them showed phosphatase activity. 2, 4-diacetyl phloroglucinol (DAPG) gene was detected in 10 isolates and pyoluteorin gene was detected in 4 isolates. Under water stress, seed treatment with P. putida (NBAII-RPF9) and P. fluorescens (PFDWD) showed its potential as plant growth promoter. The studies also indicated that stress tolerant Pseudomonas spp. may be used as plant protection agents in abiotically stressed soils.Keywords
Pseudomonas, Abiotic Stress, Tolerance, DAPG, Pyoluteorin.Full Text
References
- Ali ZSK, Sandhya V, Minakshi G, Kishore N, Rao LV, Venkateswarlu B. 2009. Pseudomonas sp. strain AKMP6 enhances tolerance of sorghum seedlings to elevated temperatures. Biol Fert Soils 46: 45–55.
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389–3402.
- Bric JM, Bostock RM, Silverstone SE. 1991. Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl Environ Microbiol. 57: 535–538.
- Delauney AJ, Verma DPS. 1993. Proline biosynthesis and osmoregulation in plants. Pl J. 4: 215–223.
- Dimkpa C, Weinand T, Asch F. 2009. Plant-rhizobacteria interactions alleviate abiotic stress conditions. Pl Cell Environ. 32: 1682–1694.
- Djedidi S, Yokoyama T, Ohkama-Ohtsu N, Risal CP, Abdelly C, Sekimoto H. 2011. Stress tolerance and symbiotic and phylogenic features of ischolar_main nodule bacteria associated with medicago species in different bioclimatic regions of tunisia. Microbes Environ. 26: 36–45.
- Egamberdieva D. 2011. Survival of Pseudomonas extremorientalalis TSAU20 and P. chlororaphis TSAU13 in the rhizosphere of common bean (Phaseolus vulgaris) under saline conditions. Pl Soil Environ. 57: 122–127.
- Egamberdieva D, Kamilova F, Validov S, Gafurova L, Kucharova Z, Lugtenberg B. 2008. High incidence of plant growth-stimulating bacteria associated with the rhizosphere of wheat grown on salinated soil in Uzbekistan. Environ Microbiol. 10: 1–9.
- Gordon SA, Weber RP. 1951. Colorimetric estimation of indole acetic acid. Pl Physiol. 26: 192–195.
- Hammer PE, Hill DD, Lam ST, Van Pée KH, Ligon JM. 1997. Four genes from Pseudomonas fluorescens that encode the biosynthesis of pyoluteorin. Appl Environ Microbiol. 63: 2147.
- Keyvan S. 2010. The effects of drought stress on yield, relative water content, proline, soluble carbohydrates and chlorophyll of bread wheat cultivars. J Anim Pl Sci. 8: 1051–1060.
- Knapp S, Schultz MJ, Van der Poll T. 2005. Pneumonia models and innate immunity to respiratory bacterial pathogens. Shock 24: 12–18.
- Mahmood M, Rahman ZA, Saud HM, Shamsuddin ZH, Subramaniam S. 2010. Influence of rhizobacterial and agrobacterial inoculation on selected physiological and biochemical changes of banana cultivar, berangan (AAA) plantlets. J Agric Sci. 2: 115–137.
- Mayak S, Tirosh T, Glick B. 2004. Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Pl Physiol Biochem. 42: 565–572.
- Naik PR, Sahoo N, Goswami D, Ayyadurai N, Sakthivel N. 2008. Genetic and functional diversity among fluorescent pseudomonads isolated from the rhizosphere of banana. Microbiol Ecol. 56: 492–504.
- Principe A, Alvarez F, Castro MG, Zachi L, Fischer SE, Mori GB, Jofr E. 2008. Biocontrol and PGPR Features in native strains isolated from saline soils of argentina. Curr Microbiol. 55: 314–322.
- Raajiijmakers JM, Weller DM, Thomashow LS. 1997. Frequency of antibiotic-producing Pseudomonas spp. in natural environments. Appl Environ Microbiol. 63: 881–887.
- Rangarajan S, Saleena LM, Vasudevan P, Nair S. 2003. Biological suppression of rice diseases by Pseudomonas spp. under saline soil conditions. Pl Soil 251: 73–82.
- Ritchie SW, Nguyan HT, Holaday AS. 1990. Leaf Water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Sci. 30: 105–111.
- Ruiz-Díez B, Fajardo S, Puertas-Mejía MA, de Felipe MR, Fernández-Pascual M. 2009. Stress tolerance, genetic analysis and symbiotic properties of ischolar_main-nodulating bacteria isolated from Mediterranean leguminous shrubs in Central Spain. Arch Microbiol. 191: 35–46.
- Saleem M, Arshad M, Hussain L, Bhatti AS. 2007. Perspective of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase in stress agriculture. J Ind Microbiol Biotech. 34: 635–648.
- Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. Sandhya V, Ali ZSk, Minakshi G, Reddy G, Venkateswarlu B. 2010. Effect of plant growth promoting Pseudomonas spp. on compatible solutes, antioxidant status and plant growth of maize under drought stress. Pl Gr Reg. 62: 21–30.
- Saravanakumar D, Kavino M, Raguchander T, Subbian P, Samiyappan R. 2011. Plant growth promoting bacteria enhance water stress resistance in green gram plants. Acta Physiol Plant. 33: 203–209.
- Shinde BM, Limaye AS, Deore GB, Laware SL. 2010. Physiological response of groundnut (Arachis hypogaea L.) varieties to drought stress. Asian J Exp Biol Sci. 65–68.
- Trabelsi D, Mengoni A, Mohammed Elarbi Aouani ME, Mhamdi R, Bazzicalupo M. 2009. Genetic diversity and salt tolerance of bacterial communities from two Tunisian soils. Ann Microbiol. 59: 25–32.
- Vyas P, Rahi P, Gulati A. 2009. Stress tolerance and genetic variability of phosphate-solubilizing fluorescent pseudomonas from the cold deserts of the trans-himalayas. Microbial Ecol. 58: 425–434.
- Weisburg WG, Barns SM, Lane DJ. 1991. 16S ribosomal DNA amplification for phylogenetic study. J Bact. 173: 697–703.
- Weller DM, Landa BB, Mavrodi OV, Schroeder KL, De La Fuente L, Bankhead SB, Molar RA, Bonsall RF, Mavrodi DV, Thomashow LS. 2007. Role of 2, 4-diacetylphloroglucinol-producing fluorescent Pseudomonas spp. in the defense of plant ischolar_mains. Pl Biol. 9: 4–20.
- Yao Y, Wu Z, Zheng Y, Kaleem I, Li C. 2010. Growth promotion and protection against salt stress by Pseudomonas putida Rs-198 on cotton. Eur J Soil Biol. 46: 49–54.
- Yun-xiu J, Xiao-dong H. 2007. Effects of plant growthpromoting rhizobacteria on the seedling growth of oat and annual ryegrass under salt stress. In: Proceedings of the International Conference on Agricultural Engineering; St. Plum Blossom Press Pvt Ltd. (Australia).